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022 _a1943-3638
040 _aCL-ChUAC
_bspa
_cCL-ChUAC
041 _aeng
_beng
_feng
245 1 0 _aEffects of enzymatic hydrolysis and ultrafiltration on physicochemical and functional properties of faba bean protein
_cEwelina Eckert ; Jay Han ; Kevin Swallow ; Zhigang Tian ; Marcela Jarpa‐Parra ; Lingyun Chen
336 _2rdacontent
_atext
_btxt
337 _2rdamedia
_aunmediated
_bn
338 _2rdacarrier
_avolume
_bnc
520 3 _aBackground and objectives Faba bean proteins isolates did not show sufficient functional properties for food applications due to poor solubility. Enzymatic treatments and ultrafiltration may improve solubility and functional properties of faba bean protein. The aim of the present work was to investigate the effect of different proteases (pepsin, trypsin, flavourzyme® 500 L, neutrase® 0.8 L), hydrolysis time and ultrafiltration technique on the physicochemical and functional characteristics of faba bean protein. Findings The protein solubility increased from 24.4% to 88.8% at pH 7 and 81.0% at pH 5 by pepsin hydrolysis (15 min). Their foaming capacity (FC) increased from 31.2% to 122.2% at pH 5 and 66.7% to 131.2% at pH 7 and the oil holding capacity (OHC) increased from 6.12 to 8.21 g/g by pepsin hydrolysis. Fraction I (Mw > 10 kDa) and II (Mw: 5–10 kDa) obtained after pepsin hydrolysis and ultrafiltration demonstrated further improved foaming and oil holding capacity and much improved emulsifying capacity. Conclusions Enzymatic hydrolysis and ultrafiltration provided a strategy to significantly improve faba bean protein solubility and functional properties. Significance and novelty Faba bean protein hydrolysates and ultrafiltration fractions are good sources of protein with excellent solubility and functionality.
650 4 _aEnzymatic hydrolysis
650 4 _aFaba bean protein
650 4 _aNutritive value
650 4 _aPhysiochemical and functional properties
650 4 _aUltrafiltration
700 1 _aEckert, Ewelina
_ecoautor
700 1 _aHan, Jay
_ecoautor
700 1 _aSwallow, Kevin
_ecoautor
700 1 _aTian, Zhigang
_ecoautor
700 1 _aJarpa‐Parra, Marcela
_ecoautor
700 1 _aChen, Lingyun
_ecoautor
773 _dNueva York, Estados Unidos
_gVolume 96, Issue 4 July/August 2019 Pages 725-741
_tCereal Chemistry [artículo de revista]
856 4 1 _uhttp://bibliorepositorio.unach.cl/handle/BibUnACh/1728
942 _2ddc
_cAREV
999 _c2366247
_d2366247